Journal: Biomedical Optics Express

Article Title: Optofluidic adaptive optics in multi-photon microscopy

doi: 10.1364/BOE.481453

Figure Lengend Snippet: The deformable phase plate (DPP). (a) Top-view drawing. Each of the transparent electrodes in the centre region can be set to an individual electric potential through connected contact pads. (b) Cross-sectional drawing and principle of operation. A voltage between an array electrode and the grounded membrane results in a membrane deformation. The locally varying optical path length leads to a wavefront modulation of an oncoming light field. (c) Photograph of the DPP naked and (d) readily assembled in its 30-mm cage-compatible housing.

Article Snippet: A novel kind of transmissive, refractive wavefront modulator that may present a well-suited alternative for such scenarios is the deformable phase plate (DPP), a transparent multi-electrode optofluidic device recently developed by some of us [ – ].

Techniques: Membrane

Journal: Biomedical Optics Express

Article Title: Optofluidic adaptive optics in multi-photon microscopy

doi: 10.1364/BOE.481453

Figure Lengend Snippet: Diagrammatic algorithm outline . Overview of critical steps shared and differing between the original and our F-SHARP variant. Functions with names ending with an exclamation mark modify their argument. FT!( … ) : Fourier transform; optimise( … ) : find the voltages that optimally reproduce a target phase mask (e.g., in Zernike basis) at given constraints . IM denotes the influence matrix, u_lim the voltage limit of the DPP electrodes (see main text).

Article Snippet: A novel kind of transmissive, refractive wavefront modulator that may present a well-suited alternative for such scenarios is the deformable phase plate (DPP), a transparent multi-electrode optofluidic device recently developed by some of us [ – ].

Techniques: Variant Assay

Journal: Biomedical Optics Express

Article Title: Optofluidic adaptive optics in multi-photon microscopy

doi: 10.1364/BOE.481453

Figure Lengend Snippet: Survey of simulated algorithm performance with respect to scatterer characteristics. Strehl ratios (blue colour scale) before correction (left column), after the 3rd (centre column) and after the 10th (right column) algorithm iteration for a layer sample, as a function of scatterer thickness, L / l s , and spectral width, σ / d 0 . Rows 1–3 correspond to the F-SHARP variants 1-B, 2-B, and 2-BI, respectively (see main text). In subfigure (a), the black dotted line marks the crossover from the ballistic to the multiple-scattering photon travel regime , and the white and black cross correspond to the turbidity levels of the left and right grey box in Fig. 4 , respectively. (h, i) Row 4 (red colour scale) shows how many of the DPP electrodes have reached their voltage limit (‘clip’), in relation to the total number of 63 electrodes. All data shown are the mean over 15 simulation runs with different random scatter masks.

Article Snippet: A novel kind of transmissive, refractive wavefront modulator that may present a well-suited alternative for such scenarios is the deformable phase plate (DPP), a transparent multi-electrode optofluidic device recently developed by some of us [ – ].

Techniques: